Envision Emtime (2024)

1. EmTime

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11 mrt 2020 · This presentation provides an overview of the following: 1. Applications available from any device from any location.

13 feb 2022 · Abstract. Analyses across imaging modalities allow the integration of complementary spatiotemporal information about brain development, ...
Analyses across imaging modalities allow the integration of complementary spatiotemporal information about brain development, structure and function. However, systematic atlasing across modalities is limited by challenges to effective image alignment. We combine highly spatially resolved electron microscopy (EM) and highly temporally resolved time-lapse fluorescence microscopy (FM) to examine the emergence of a complex nervous system in C. elegans embryogenesis. We generate an EM pseudo time series at four classic developmental stages and create a landmark-based co-optimization algorithm for cross-modality image alignment, which handles developmental heterochrony among datasets to achieve accurate single-cell level alignment. Synthesis based on the EM series and time-lapse FM series carrying different cell-specific markers reveals critical dynamic behaviors across scales of identifiable individual cells in the emergence of the primary neuropil, the nerve ring, as well as a major sensory organ, the amphid. Our study paves the way for systematic cross-modality analysis in C. elegans and demonstrates a powerful approach that may be applied broadly. Highlights ### Competing Interest Statement The authors have declared no competing interest.

We generate an EM time series at four classic developmental stages and create a landmark-based co-optimization algorithm for cross-modality image alignment, ...
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Analyses across imaging modalities allow the integration of complementary spatiotemporal information about brain development, structure, and function. However, systematic atlasing across modalities is limited by challenges to effective image alignment. ...

6 jun 2022 · We generate an EM time series at four classic developmental stages and create a landmark-based co-optimization algorithm for cross-modality ...
A landmark-based cross-modality alignment method robust to variation in landmark sets is applied to annotate an EM time series of Caenorhabditis elegans embryonic development as a community resource.

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Physicians and patients want a better model of health care. They envision a system in which all involved use the Web as a platform to share information, deliver care and build communities around medical interests and health goals.